1,3-diamino-5-phenylphenazinium chloride

Chemical structure

1,3-diamino-5-phenylphenazinium chloride

Physical state

Yellow water-soluble dye

Application

Used in cell viability and proliferation assays

Mechanism of action

Converted to a formazan product by mitochondrial reductases in living cells

Measurement

Formazan product can be measured spectrophotometrically to assess metabolic activity of cells

Usage

Widely used in biomedical research and pharmaceutical testing

Purpose

Evaluate the effects of drugs, toxins, and environmental factors on cell viability and proliferation

Safety precautions

Toxic if ingested or inhaled, can cause skin and eye irritation

Handling

Proper safety measures and protocols should be followed when working with this chemical

General Description

1,3-diamino-5-phenylphenazinium chloride, also known as MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), is a yellow water-soluble dye often used in cell viability and proliferation assays. It is a redox indicator that is converted to a formazan product by mitochondrial reductases in living cells. The formazan product can be measured spectrophotometrically to assess the metabolic activity of the cells. MTT is widely used in biomedical research and pharmaceutical testing to evaluate the effects of drugs, toxins, and environmental factors on cell viability and proliferation. However, caution should be exercised in handling MTT as it is toxic if ingested or inhaled, and can cause skin and eye irritation. Proper safety measures and protocols should be followed when working with this chemical.